Use the -f or --fasta flag to specify the path to your FASTA file. For ENSEMBL and Gencode files, the -t or --taxonomy flag is required. This flag should provide the organism’s taxonomy (e.g., ...

GFF files have a somewhat complex structure, but by using this annotation, you can extract arbitrary sequences from a FASTA file. Here, as a test, I will write code that extracts all CDS, full-length ...