MicroRNAs (miRNAs) play key roles in development and disease, and have great biomarker potential. However, because miRNA expression is highly cell-type specific, identifying miRNA biomarkers from ...

Based on single-stranded DNA library method, we established an efficient workflow to parallelly construct targeted genomic and epigenomic sequencing libraries from a small amount of DNA. We applied ...

The previous article in this series, From Conventional Mutagenesis to Complex, Rationally Designed Mutant Libraries, described how conventional site-directed mutagenesis methods have been improved to ...