PCR is a widely employed and indispensable tool for an extensive and ever-growing number of molecular applications 1. These applications include protocols in diverse fields such as biomedical research ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

One of the critical prerequisites for efficient PCR-based diagnosis in a molecular laboratory is accurate primer design. The conventional primer design process relies on rule-based algorithms to ...